HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS REGISTER		Author Keyword(s) Vol Page [Advanced]

This Article Full Text Full Text (PDF) All Versions of this Article: thx.2005.049403v1 61/2/134    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this link to a friend Similar articles in this journal Similar articles in PubMed Add article to my folders Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peters, M Search for Related Content PubMed PubMed Citation Articles by Peters, M Topic Collections Related Article

Inhalation of stable dust extract prevents allergen induced airway inflammation and hyperresponsiveness

¹ Department of Experimental Pneumology, Ruhr-University Bochum, Bochum, Germany
² Division of Structural Biochemistry, Forschungszentrum Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany
³ Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College, London, UK
⁴ Klinik für Kinder- und Jugendmedizin, St Josef-Hospital, Bochum, Germany
⁵ Kinderspital Schwarzach, Schwarzach, Austria
⁶ Institute of Occupational and Environmental Medicine, University of Munich, Munich, Germany
⁷ Institute of Social and Preventive Medicine, Basel, Switzerland
⁸ Dr von Hauner Children’s Hospital, Munich, Germany

Correspondence to:
Correspondence to:
Dr M Peters
Department of Experimental Pneumology, Ruhr-University Bochum, Bürkle-de-la-Camp-Platz 1, 44789 Bochum, Germany; marcus.peters{at}rub.de

Background: Recent epidemiological studies have shown that growing up on a traditional farm provides protection from the development of allergic disorders such as hay fever and allergic asthma. We present experimental evidence that substances providing protection from the development of allergic diseases can be extracted from dust collected in stables of animal farms.

Methods: Stable dust was collected from 30 randomly selected farms located in rural regions of the Alps (Austria, Germany and Switzerland). The dust was homogenised with glass beads and extracted with physiological sodium chloride solution. This extract was used to modulate immune response in a well established mouse model of allergic asthma.

Results: Treatment of mice by inhalation of stable dust extract during sensitisation to ovalbumin inhibited the development of airway hyperresponsiveness and airway eosinophilia upon challenge, as well as the production of interleukin 5 by splenocytes and of antigen specific IgG₁ and IgE. Dust extract also suppressed the generation of human dendritic cells in vitro. The biological activity of the dust extract was not exclusively mediated by lipopolysaccharide.

Conclusions: Stable dust from animal farms contains strong immune modulating substances. These substances can interfere with the development of both cellular and humoral immunity against allergens, thus suppressing allergen sensitisation, airway inflammation, and airway hyperresponsiveness in a murine model of allergic asthma.

Abbreviations: AHR, airway hyperresponsiveness; BAL, bronchoalveolar lavage; IFN-, interferon ; IL, interleukin; LPS, lipopolysaccharide; PBS, phosphate buffered saline

Keywords: allergen sensitisation; animal model; asthma; hygiene hypothesis

Related Article

Airwaves

Wisia Wedzicha
Thorax 2006 61: 93. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				E. von Mutius Asthma and Allergies in Rural Areas of Europe Proceedings of the ATS, July 1, 2007; 4(3): 212 - 216. [Abstract] [Full Text] [PDF]