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Decreased expression of interleukin 13 in human lung emphysema

¹ INSERM U 408, Hôpital Bichat-Claude Bernard, Paris, France
² Service de Pneumologie, Hôpital Bichat-Claude Bernard, Paris, France
³ Service de Physiologie-Explorations Fonctionnelles, Hôpital Bichat-Claude Bernard, Paris, France
⁴ Service de Biochimie A, Hôpital Bichat-Claude Bernard, Paris, France
⁵ Service de Pneumologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris, France
⁶ Service de Chirurgie Thoracique, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris, France

Correspondence to:
Correspondence to:
Dr A Boutten
Laboratoire de Biochimie A, Hôpital Bichat-Claude Bernard, 46 rue Henri Huchard, 75870 Paris, Cedex 18, France; anne.boutten{at}bch.ap-hop-paris.fr

Background: The overexpression of interferon (IFN) or interleukin (IL)-13 in the adult murine lung induces the development of changes that mirror human lung emphysema.

Methods: IL-13 and IFN expression was determined in lung samples from five groups of patients: severe emphysema without ₁-antitrypsin deficiency (SE+, n = 10); severe emphysema with ₁-antitrypsin deficiency (SE–, n = 5); mild localised emphysema (ME, n = 8); non-emphysema smokers (NE-S, n = 9), and non-emphysema non-smokers (NE-NS, n = 11). Lung IL-13 and IFN mRNA were analysed by RT-PCR. Lung concentrations of IL-13 protein were assessed by ELISA.

Results: The expression of IFN mRNA was similar in patients with or without emphysema. IL-13 mRNA was markedly decreased in the SE+ group compared with the SE– (p = 0.04), ME (p = 0.02), and non-emphysema groups (p = 0.01). IL-13 mRNA correlated with forced expiratory volume in 1 second (r = 0.5, p = 0.04) and arterial oxygen tension (r = 0.45, p = 0.03) in emphysema patients. In contrast to the non-emphysematous lung, IL-13 protein was below the detection limit of the assay in most emphysematous lung homogenates.

Conclusion: The lung IL-13 content is reduced in patients with severe emphysema without ₁-antitrypsin deficiency.

Keywords: emphysema; interleukin 13; interferon

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